Journal: Journal of Cellular and Molecular Medicine

Article Title: TREM2 expression promotes liver and peritoneal M2 macrophage polarization in mice infected with Schistosoma japonicum

doi: 10.1111/jcmm.17842

Figure Lengend Snippet: Trem2 deletion increases F4/80 + CD86+ cells in peritoneal macrophages. (A) TREM2 expression is upregulated in peritoneal macrophages from mice infected with Schistosoma japonicum . The number of TREM2+ cells in F4/80 + CD11b + peritoneal macrophages was determined by flow cytometry and compared to uninfected controls. * p < 0.05, ** p < 0.01, *** p < 0.001. (B) The number of F4/80 + CD86+ cells in total peritoneal macrophages obtained from wild‐type or Trem2 −/− mice with or without IL4/13 stimulation was determined by flow cytometry. ** p < 0.01. (C) The number of F4/80 + CD206+ cells in total peritoneal macrophages obtained from wild‐type or Trem2 −/− mice with or without IL4/13 stimulation was determined by flow cytometry. * p < 0.05. All results are relative to their respective controls.

Article Snippet: For this, the cells were incubated with PE conjugated antibody against mouse F4/80 (02922–60, Biogems), PE‐Cy7 conjugated antibody against mouse CD11b (03221–77, Biogems), APC conjugated antibody against mouse CD86 or CD206 (17–0862, 17–2061, eBioscience) and FITC conjugated antibody against mouse TREM2 (ab119852, Abcam).

Techniques: Expressing, Infection, Flow Cytometry